In vitro Callus Induction and Shoot Regeneration in Hollyhocks (Althaea digitata)

Zeinab Hosseini, Hamid Reza Ghasempour, Danial Kahrizi, Leila Akbari

Abstract


Althaea digitata (Boiss.) is considered as one of the valuable medicinal plants in the world which is belong to Malvaceae family. Tissue culture techniques have been extensively used for improving and studying of traits in medicinal plants. The present study was conducted to induce callus from shoot, root and leaf explants and shoot regeneration in A. digitata. Explants were cultured on Murashige and Skoog (MS) medium supplement with different concentrations and combinations of 6-benzylaminopurine [BAP] (0, 1, 3, and 5 mg L-1), Kinetin [KIN] (0.1 mg L-1), naphthalene acetic acid [NAA] (0.1 mg L-1) and 2,4-dichlorophenoxyacetic acid [2,4-D] (0, 2, 5 and 10 mg L-1). Best results for callus induction was observed in shoot tip explants on MS+5.0 mg L-1 2, 4-D+0.1 mg L-1, KIN (82.98%). After callus initiation, it was immediately transferred to MS medium containing BAP and NAA. Incubation for about 10-12 weeks on the same culture medium resulted shoot buds initiation from the callus. The Maximum regeneration frequency was observed on MS medium containing 0.1 mg L-1 NAA and 1.0 mg L-1 BAP. The method can be applied in transformation experiments.

 

Keyword: 2,4-D, 2,4, Dichlorophenoxyacetic Acid; BAP, 6-Benzyl Amino Purine; MS, Murashige and Skoog NAA, Naphthalene Acetic Acid


Full Text:

PDF

References


Aftab, F., Iqbal, J., 1999. Plant regeneration from protoplastas derived from cell suspension of adventive somatic embryos in sugarcane (Saccharum sp.) hybrid cv. Col-54 and cv. CP- 43/ 33. Plant Cell, Tissue and Organ Culture, 56 (3): 155–162.

Ameri, A., Heydarirad, G., MahdaviJafari, J., Ghobadi, A., Rezaeizadeh, H., Choopani, R., 2015. Medicinal plants contain mucilage used in traditional persian medicine (TPM). Pharmaceutical Biology, 53(4): 615-623.

Ameri, A., Heydarirad, G., Rezaeizadeh, H., Choopani, R., Ghobadi, A., Gachkar, L., 2016. Evaluation of efficacy of an herbal compound on dry mouth in patients with head and neck cancers: A randomized clinical trial. Journal of Evidence-Based Complementary and Alternative Medicine, 21 (1): 30-33.

Arous, S., Boussaïd, M, Marrakchi, M., 1998. Preliminary research about in vitro regeneration of tunisian pepper Capsicum annuum L. In XthEucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant. Avignon-France, September 7-11: 187-189.

Atal, C. K., Kapur, B. M., 1982. Cultivation and utilization of medicinal plants. Jammu-Tawi, India: Regional Research Laboratory, Council of Scientific and Industrial Research.

Bajguz, A., Piotrowska, A., 2009. Phytochemistry, 70 (8): 957-969.

Baskaran, P., Raja Rajeswari, B., Jayabalan, N., 2006. Development of an in vitro regeneration system in sorghum [Sorghum bicolor (L.) Moench] using root transverse thin cell layers (tTCLs). Turkish Journal of Botany, 30 (1): 1-9.

Bohidar, S., Thirunavoukkarasu, M., Rao, T. V., 2008. Effect of plant regulators on in vitro micropropagation of Garden Rue (RutaGraveolens L.). International Journal of Integrative Biology, 3(1): 36-43.

Eisenbeiss, M., Kreis, W., Reinhard, E., 1999. Cardenolide biosynthesis of light and dark grown Digitalis lanata shoot culture. Plant Biology and Biochemistry, 37 (1): 13-24.

Emma, M. J., Sevdalina, A., Verkleij, A. C. J., 2005. Callus induction and plant regeneration in the metallophyte Silene vulgaris (Caryophyllaceae). Plant Cell, Tissue and Organ Culture, 80 (1): 25-31.

Faccila, S. C., 1990. A sourcebook of edible plants. Kampong Publications.

Fitch, M. M., Moore, P. H., 1993. Long-term culture of embryogenic sugarcane callus. Plant Cell, Tissue and Organ Culture, 32 (3): 335–343.

Gang, Y. Y., Du, G. S., Shi, D. J., Wang, M. Z., Li, X. D., Hua, Z. L., 2003. Establishment of in vitro regeneration system of the Atrichum mosses. Acta Botanica Sinica, 45(12): 1475-1480.

Ghasempour, H. R., Soheilikhah, Z. h., Zebarjadi, A. R., Ghasempour, S., Karimi, N., 2014. In vitro micropropagation, callus induction and shoot regeneration in safflower L. cv. Lesaf'. Iranian Journal of Plant Physiology, 4 (2): 999-1004.

Gill, R., Malhotra, P. K., Gosal, S. S., 2006. Direct plant regeneration from cultured young leaf segments of sugarcane. Plant Cell, Tissue and Organ Culture, 84 (2): 227-231.

Hanumanaika, R. N., Venkatarangaiah, K., 2008. Plant regeneration from callus culture of Clematis gourianaRoxb. – a rare medicinal plant. Turkish Journal of Biology, 32 (2): 99-103.

Heywood, V. H., 1978. Flowering plants of the world. London: Oxford University Press.

Hutchinson, J., 1964. The genera of flowering plants (Angiospermae). Dicotyledones, Oxford: Oxford University Press, No. C/852.13 H8/v. 1.

Hutchinson, J., 1973. The families of flowering plants (Angiospermae) Dicotyledones. Oxford: Oxford University Press.

Iljin, M. M., 1949. Flora URSS. Mosqua-Leningrad.

Jafari, A., Kahrizi, D., Mansouri, M., 2016. Effects of plant growth regulators and explant on callus induction in pennyroyal (Menthapulegium L.). Biharean Biologist, 10 (2): 134-136.

Kim, H. S., 2005. Do not put too much value on conventional medicines. Journal of Ethnopharmacology, 100 (1):37–39.

Li. J., Jain, M., Vunsh, R., Vishnevetsky, J., Hanania, U., Flaishman, M., Perl, A., Edelman, M., 2004. Callus induction and regeneration in Spirodela and Lemna. Plant Cell Reports, 22 (7): 457-464.

Mohapatra, A., Rout, G. R., 2004. Effect of cytokinin and auxin on micropropagation of Geoderum purpureum R. BR. Journal of Applied Horticulture, 6(2): 27-29.

Mangat, B. S., Roy M. K., 1986. Tissue culture and plant regeneration of kora. Plant Science, 47: 57-61.

Murashige, T., Skoog, F., 1962. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. plant, 15: 473-497.

Nasır, E., Ali, S. I., 1979. Flora of West Pakistan. Karachi: Department of Botany, University of Karachi.

Nalawade, S. M., Sagare, A. P., Lee, C. Y., Kao, C. L. Tsay. H. S., 2003. Studies on tissue culture of Chinese medicinal plant resources in Taiwan and their sustainable utilization. Botanical Bulletin of Academia Sinica, 44: 79-98.

Pakseresht, G., Kahrizi, D., Mansouri, M., Ghorbani, T., Kazemi, N., 2016. Study of callus induction and cell culture to secondary metabolite production in HyssopusofficinalisL. Journal of Reports in Pharmaceutical Sciences, 5 (2): 104-111.

Pierik, R. L. M., 1987. In vitro culture of higher plants. Springer Science and Business Media. p. 344.

Rao, A. Q., Hussain, S. S., Shahzad, M. S., Bokhari, S. Y. A., Raza, M. H., Rakha, A., Majeed, A., Shahid, A. A., Saleem, Z., Husnain, T., Riazuddin, S., 2006. Somatic embryogenesis in wild relatives of cotton (Gossypium spp.). Journal of Zhejiang University. (Science B), 7(4): 291-298.

Rout, G. R., Samantaray, S., Das, P., 2000. In vitro manipulation and propagation of medicinal plants. Biotechnology Advances, 18 (2): 91-120.

Sen, M. K., Nasrin, S., Rahman, S., Jamal, A. H., 2014. In vitro callus induction and plantlet regeneration of Achyranthesaspera L., a high value medicinal plant. Asian Pacific Journal of Tropical Biomedicine, 4(1): 40-46.

Shrivastava, S., Dubey, P. K., 2007. In vitro callus induction and shoot regeneration in Withania somnifera Dunal. International Journal of Biochemistry, 24, 10-19.

Skoog, F., Armstrong, D. J., 1970. Cytokinin annual review. Plant Physiology, 21: 359-384.

Soorni, J., Kahrizi, D., 2015. Effect of genotype, explant type and 2,4-D on cell dedifferentiation and callus induction in cumin (Cuminum cyminum L.) medicinal plant. Journal of Applied Biotechnology Reports, 2 (3): 265-270.

Taimori, N., Abdossi, V., Papzan, A. H., 2016. Cell dedifferentiation, callus induction and somatic embryogenesis in Crataegus spp. Cellular and Molecular Biology, 62 (11): 100-107.

Ul Hassan, H., Murad, W., Tariq, A., Ahmad, A., 2014. Ethnoveterinary study of medicinal plants in Malakand Valley, District Dir (Lower), Khyber Pakhtunkhwa, Pakistan. Irish Veterinary Journal, 67 (1):6.

Uzunhisarcikli, M. E., Vural, M., 2012. The taxonomic revision of Alcea and Althaea (Malvaceae) in Turkey. Turkish Journal of Botany, 36 (6): 603-636.

Vargas, T. E., Mejias, A., Oropeza, M., Garcia, E., 2004. Plant regeneration of Anthurium andreanum cv Rubrun. Electronic Journal of Biotechnology, 7 (3): 282-286.

Yang, J., Gong, Z. C., Ten, X., 2008. Induction of callus and extraction of alkaloid from Yi Mu Cao (Leonurus heterophylus Sw.) culture. African Journal of Biotechnology, 7 (8): 1157-1162.

Zargaran, A., Ahmadi, S., Daneshamouz, S., Mohagheghzadeh, A., 2012. Ancient Persian pharmaceutical vessels and tools in Iranian archaeological museums. Pharmaceutical Historian, 42 (4):68–71.

Zinhari, Z., Pourseyedi, Sh., Zolala, J., 2016. Callus induction and direct shoot regeneration in Lepidiumdraba L. Explants. Journal of Agricultural Biotechnology, 8 (2): 31-51.


Refbacks

  • There are currently no refbacks.