In vitro Callus Induction and Shoot Regeneration in Hollyhocks (Althaea digitata)

Zeinab Hosseini, Hamid Reza Ghasempour, Danial Kahrizi, Leila Akbari


Althaea digitata (Boiss.) is considered as one of the valuable medicinal plants in the world which is belong to Malvaceae family. Tissue culture techniques have been extensively used for improving and studying of traits in medicinal plants. The present study was conducted to induce callus from shoot, root and leaf explants and shoot regeneration in A. digitata. Explants were cultured on Murashige and Skoog (MS) medium supplement with different concentrations and combinations of 6-benzylaminopurine [BAP] (0, 1, 3, and 5 mg L-1), Kinetin [KIN] (0.1 mg L-1), naphthalene acetic acid [NAA] (0.1 mg L-1) and 2,4-dichlorophenoxyacetic acid [2,4-D] (0, 2, 5 and 10 mg L-1). Best results for callus induction was observed in shoot tip explants on MS+5.0 mg L-1 2, 4-D+0.1 mg L-1, KIN (82.98%). After callus initiation, it was immediately transferred to MS medium containing BAP and NAA. Incubation for about 10-12 weeks on the same culture medium resulted shoot buds initiation from the callus. The Maximum regeneration frequency was observed on MS medium containing 0.1 mg L-1 NAA and 1.0 mg L-1 BAP. The method can be applied in transformation experiments.


Keyword: 2,4-D, 2,4, Dichlorophenoxyacetic Acid; BAP, 6-Benzyl Amino Purine; MS, Murashige and Skoog NAA, Naphthalene Acetic Acid

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