Seed Treatment to Overcome Autotoxicity of Forage Rape

Farid Golzardi, Nima Aghamollaei


In order to evaluate the effects of different priming treatments on reducing autotoxicity potential of forage rape, an experiment was conducted with a factorial arrangement based on completely randomized design with four replications at Islamic Azad University of Karaj during 2014. Factors of experiment consisted of forage rape extract in three levels (aerial parts, underground tissues, and combination of different parts) and various seed priming methods in six levels (control, hydro-priming, osmo-priming with PEG, hormonal priming with gibberellic acid, potassium nitrate priming, and priming by salicylic acid. The studied traits according to ISTA (International Seed Testing Association)instruction were: rate and percentage of germination, length of shoot and root, fresh and dry weight of seedling. The results showed that the extracts of various parts of forage rape inhibits the seedling growth in different ways by affecting its germination traits. The lowest and highest percentage of seed germination was observed in extracts of underground parts and aerial organs, respectively. Different priming treatments also have various inhibiting role for reducing autotoxicity potential of forage rape. As regards, hydro-priming and osmo-priming with PEG were the most influential methods of priming. Germination rate of seeds primed with salicylic acid was zero.It seems that salicylic acid has inhibitory effects on seed germination of forage rape. Germination rate of seeds primed with gibberellic acid (1.29 day-1) and potassium nitrate (1.57 day-1) was less than control treatment (2.19 day-1). According to results, hydro-priming or osmo-priming with PEG could be a suitable tool for reducing autotoxicity potential of forage rape and improving germination characteristics, particularly for monoculture systems.


Keyword: Germination rate, Hydro-priming, Osmo-priming, Seedling weight

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